Ready to get quantitative? When the Base Reference Set isn’t quite enough, this extended kit pushes the envelope of performance characterization. When fluorescence images are collected using both the Reference Targets and the solid state emitter in the Radiometric Target, a more complete analysis of the system sensitivity can be developed. Our engineering team is currently developing protocols to use this kit to determine the absolute fluorescence yield of imaging systems, and we’d be happy to work with you through this process.
The reference targets in this kit are designed to mimic ICG fluorescence in the presence of tissue optical properties. The concentration sensitivity target provides a method to determine an imaging system’s range of linearity as well as an estimate for the noise floor and saturation point. The depth sensitivity target contains 1uM ICG-equivalent material at depths of 0.5-6mm under non-fluorescent tissue-mimicking material, which simulates in vivo imaging environments. The fluorescence resolution target uses a negative USAF1951 test target and 1uM ICG-equivalent material to provide an estimate of the imaging system’s optimal resolution to determine the resolvable feature size in fluorescence mode. The radiometric target provides a stable reference with know calibrated radiant output.
The test targets in this set can be analyzed using our web portal at FGS.QUELimaging.com. We are currently enrolling Beta testers for this site. A brief overview of the current features are available in the video below. The aim of this service is to provide system developers and researchers a reliable, repeatable and time-efficient method to analyze fluorescence images of these reference targets.