This reference target is an ideal solution understand the resolution of your fluorescence imaging system. This target uses a negative USAF1951 chart with progressively smaller line-pairs and 1uM ICG-equivalent material to provide an estimate of the imaging system’s optimal resolution to determine the resolvable feature size in fluorescence mode. A contrast transfer function can be estimated by analyzing images of this target.
Fluorescence images of this target can be analyzed using our web portal at FGS.QUELimaging.com. We are currently enrolling Beta testers for this site. A brief overview of the current features are available in the video below. The aim of this service is to provide system developers and researchers a reliable, repeatable and time-efficient method to analyze fluorescence images of these reference targets.