This kit is a great solution to collect performance benchmarking data of your fluorescence imaging system. It incorporates Methylene Blue fluorescence in the presence of tissue optical properties. The concentration sensitivity target provides a method to determine an imaging system’s range of linearity as well as an estimate for the noise floor and saturation point, with concentrations ranging from 1 nM to 100 uM Methylene Blue. The depth sensitivity target contains 100 uM Methylene Blue embedded in resin at depths of 0.5-6mm under non-fluorescent tissue-mimicking material, which simulates in vivo imaging environments. The fluorescence resolution target uses a negative USAF1951 test target and 100uM Methylene Blue material to provide an estimate of the imaging system’s optimal resolution to determine the resolvable feature size in fluorescence mode.