(Limited) Methylene Blue reference set


This kit is a LIMITED RELEASE! Once the current inventory is sold, the kit will not be available for standard order.

Custom orders are always an option and we look forward to discussing your specific needs.


  1. Methylene Blue concentration sensitivity target
    • 8 wells 1nM – 100 uM Methylene Blue material
    • 1 negative control (0 nM)
    • All wells have base optical coefficients:
      • µₐ = 0.035 mm⁻¹ at 700nm
      • µ′ₛ = 0.30 mm⁻¹ at 700nm
  2. Methylene Blue depth sensitivity target
    • Top: 0.5-6mm tissue-equivalent material non-fluorescent material
      • µₐ = 0.018 mm⁻¹ at 700nm
      • µ′ₛ = 2.1 mm⁻¹ at 700nm
    • Bottom: 8 wells 100 uM Methylene Blue material, 1 negative control well
      • µₐ = 0.035 mm⁻¹ at 700nm
      • µ′ₛ = 0.30 mm⁻¹ at 700nm
    • Negative control has 6mm of top material
  3. Methylene Blue resolution target
    • Group 0 through Group 7
    • 1.5 mm thick low-reflective glass
    • 100 uM Methylene Blue material backing
      • µₐ = 0.035 mm⁻¹ at 700nm
      • µ′ₛ = 0.30 mm⁻¹ at 700nm

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In stock


This kit is a great solution to collect performance benchmarking data of your fluorescence imaging system. It incorporates Methylene Blue fluorescence in the presence of tissue optical properties.  The concentration sensitivity target provides a method to determine an imaging system’s range of linearity as well as an estimate for the noise floor and saturation point, with concentrations ranging from 1 nM to 100 uM Methylene Blue. The depth sensitivity target contains 100 uM Methylene Blue embedded in resin at depths of 0.5-6mm under non-fluorescent tissue-mimicking material, which simulates in vivo imaging environments. The fluorescence resolution target uses a negative USAF1951 test target and 100uM Methylene Blue material to provide an estimate of the imaging system’s optimal resolution to determine the resolvable feature size in fluorescence mode.


Additional information

Weight 205 g
Dimensions 207 × 92 × 42 mm